基于GIS的喀斯特流域石漠化综合防治探讨——以贵州织金县洗马河流域为例

喀斯特石漠化是在喀斯特脆弱生态环境下,人类不合理的社会经济活动,造成植被破坏、水土流失、岩石逐渐裸露、土地生产力衰退丧失的演变过程,严重制约了区域社会经济的可持续发展。洗马河流域是织金洞国家级重点风景名胜区核心主成部分,主要岩性为灰岩和白云岩,喀斯特十分发育,是典型的石漠化区域,占流域面积近70%,人地矛盾十分突出。项目通过GIS与遥感技术为手段,进行石漠化调查和防治工程的布置,从而达到对该流域的石漠化防治,挽救该区域人类生存条件、防治水土流失、对国家重点风景名胜区的保护,同时对其它喀斯特区域石漠化的综合防治起着示范作用。     

腹泻抗原融合基因CTB-CS3对胡萝卜的遗传转化

CS3是CFA(产肠毒素型大肠杆菌引起腹泻时产生两类毒性因子之一)阳性株的保护性抗原。临床研究结果表明,以CS3作为疫苗可有效地防止由相关细菌导致的腹泻(董自正等,     

天然林保护工程中森林管护存在的问题及对策

森林管护是天然林保护工程建设的重要内容,天然林保护工程实施方案和工程管理办法对森林管护有严格的规定和要求。阐述天然林保护工程中森林管护工作现状和存在的主要问题,探讨解决问题的对策措施。     

利用棉绳采集口腔液监测猪伪狂犬病方法的应用分析

为分析使用棉绳采集口腔液监测猪场伪狂犬病（PR）方法的科学性,本研究拟通过实验室水平、动物试验及临床应用3个方面对使用棉绳采集口腔液监测猪场PR这一方法开展应用分析。首先对采样条件进行优化,并通过模拟试验测定棉绳对伪狂犬病病毒（PRV）的释放能力,通过动物试验测定病毒感染后检出时间,最后对临床样品进行检测分析。结果表明：使用直径为1.0 cm的棉绳,在早上喂料前采集口腔液样品,采样时间为20~30 min,可采集到更能满足试验要求的口腔液。病毒释放能力测定显示,棉绳对PRV的释放能力为50%左右,使用棉绳采集口腔液可检测到1个TCID₅₀/0.1 mL的病毒含量。动物试验检测发现,猪群在感染后28 d中除第5天外,口腔液中病原含量均高于鼻拭子中病原含量,口腔液检测效果优于鼻拭子,且口腔液中病毒的检出时间（感染后第1天）早于血液中抗体转阳时间（感染后第7天）。临床样品检测分析结果表明,PRV疫苗免疫后也可通过口腔液检测到疫苗毒,并且存在无法通过口腔液检测感染PRV野毒后稳定猪中带毒的情况,因此口腔液检测方法应结合gE抗体检测,才可综合判断猪群是否为感染群体。综上,本研究优化了口腔液采集方法,并测定了棉绳对口腔液的释放能力和感染后检出时间,表明口腔液可作为较好的监测猪群PR的手段;口腔液监测方法需结合gE抗体检测来综合判断猪群是否为感染群体。     

农业院校非英语专业学生口语僵化的激活实验研究

非英语专业大学生英语口语中的语言僵化是一个普遍现象。本文通过教学实践和调查具体分析了大学生口语僵化的原因，从而制定出了口语激活策略，从教学理念、教学方法和考试模式三个方面进行改革。通过一年的教学实践，学生在口语表达时的流利程度、语法词汇的正确使用和交际能力方面都有了显著的提高，而且综合英语能力也有很大提高。实验结果证明，非英语专业大学生的口语僵化是可以被激活的。     

Inflammation and wound healing in cats with chronic gingivitis/stomatitis after extraction of all premolars and molars were not affected by feeding of two diets with different omega‐6/omega‐3 polyunsaturated fatty acid ratios

Feline chronic gingivitis/stomatitis (FCGS) is a painful inflammatory disease in cats. Extraction of teeth, including all premolars and molars, has been shown to be the therapy of choice in cats not responding sufficiently to home care (e.g. tooth brushing) and/or medical treatment (corticosteroids and/or antibiotics). In this study, we hypothesize that a cat food with an omega‐6 polyunsaturated fatty acid (ω6 PUFA) to ω3 PUFA ratio of 10:1 reduces inflammation of the FCGS and accelerates soft tissue wound healing of the gingiva after dental extractions, compared to a cat food with a ω6:ω3 PUFA ratio of 40:1. The cats were fed diets with chicken fat and fish oil as sources of fatty acids. In one diet, part of the fish oil was replaced by safflower oil, resulting in two diets with ω6:ω3 PUFA ratios of 10:1 and 40:1. This double‐blinded study in two groups of seven cats revealed that dietary fatty acids influence the composition of plasma cholesteryl esters and plasma levels of inflammatory cytokines. The diet with the 10:1 ratio lowered PGD₂, PGE₂ and LTB₄ plasma levels significantly, compared to the diet with the 40:1 ratio (p = 0.05, p = 0.04, and p = 0.02 respectively). However, feeding diets with dietary ω6:ω3 PUFA ratios of 10:1 and 40:1, given to cats with FCGS for 4 weeks after extraction of all premolars and molars, did not alter the degree of inflammation or wound healing.     

Protection of Fenneropenaeus chinensis (Osbeck, 1765) against the white spot syndrome virus using specific chicken egg yolk immunoglobulins by oral delivery

Two kinds of specific chicken egg yolk immunoglobulins (IgYs), IgY‐WSSV and IgY‐VP28, were, respectively, raised against the 2 mM binary ethylenimine (BEI)‐inactivated white spot syndrome virus (WSSV) and a principal envelope protein VP28. The activity of purified specific IgYs was stable under the conditions of 20–70 °C, pH 3.0–10.0 and 0–700 g L^?1 sucrose solution. In the neutralization assay, these high‐affinity IgY antibodies can specifically bind with the virus particles to protect shrimp (Fenneropenaeus chinensis) against WSSV infection. After oral delivery for 20 days, the IgY‐WSSV exerted a higher protection effect (RPS: 71.5%) than IgY‐VP28 (RPS: 63.7%). Moreover, an increase in RPS (79.2%) was found on addition of IgY‐WSSV:VP28 (0.1% IgY‐VP28 plus 0.2% IgY‐WSSV). This may indicate that neutralization of WSSV refers to the multiple‐hit model. By time‐course study of the levels of the specific IgYs in vivo, the data showed that the titre was enhanced to a relatively high level (P/N=8.35±0.45) at 3 days post administration, declined slightly (P/N=7.13±1.01) at 7 days post administration and then remained stable for further investigation. The stable antibody level potentially contributes towards blocking a large number of WSSV particles from entering and infecting on the major tissues at the early and late stages after challenge in shrimp.     

“Do Not Let the Grange Die!” Michigan Grangers' Struggle for Continued Relevance in the Twenty-First Century

ABSTRACT

The Grange was founded during a time when farmers were the majority of the population and secrecy was a means of survival. More than one hundred thirty-five years later, those characteristics are still strongly associated with it. Yet there is a growing consciousness among many members that the Grange has to become better at promoting itself among nontraditional constituencies-or it will die. This article explores the Grange experiences of five members. All have been profoundly affected by their membership. All are struggling with the need to reconcile loyalty to tradition with the need to change and adapt. In sharing their thoughts and feelings, the study has the opportunity to consider what the Grange's past was, what its present is, and what its future may become.     

基于资源资产化管理的大连市海洋综合管理新体制构建探讨

随着大连市海洋资源开发利用的发展，其产生的一系列问题逐步显现。而要解决这些问题就要改革完善大连市的海洋管理体制。在明确了改革基本要求的基础上，探讨了大连市海洋管理体制改革的理论依据，论述了大连市海洋管理体制改革的基本思路，最后构建了大连市海洋综合管理新体制。